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Buljongfortynning, spesialplater

MICRONAUT-S Carbapenemases

2-test plate MICRONAUT-S Carbapenemases Detection system

Phenotypic confirmation of KPC, MBL as well as OXA-48-like type D carbapenemases in Enterobacterales. The new inhibitor combination meropenem-avibactam and determination of high-level temocillin resistance improves phenotypic detection of OXA-48-like enzymes in enterobacteria.

By selection of the concentration ranges of meropenem as mono compound (0.06 – 128 mg/L) and the inhibitor combinations meropenem-3-amino-phenyl-borate (3-APB), meropenem-EDTA and meropenem-avibactam (each 0.031 – 32 mg/ml), the detection of type A carbapenemases (e.g. KPC), metallo-β-lactamases and OXA-48-like type D carbapenemases is possible when the meropenem screening cut-off concentration for enterobacteria is exceeded1.

1EUCAST guidelines for detection of resistance mechanisms and specific resistances of clinical and/or epidemiological importance. EUCAST Version 2.0, July 2017.

MICRONAUT-S ß-Lactamases

  • MIC determination of antibiotics and antibiotic combinations against all relevant gram-negative bacteria (Enterobacterales, AeromonadaceaePseudomonadaceae etc.)
  • ESBL: 3 extended spectrum cephalosporins and their combination with clavulanic acid
  • Amp-C:  3 extended spectrum cephalosporins and their combination with 3-Amino-Phenyl-Borate (3-APB)
  • KPC:  MIC determination of meropenem as a mono substance and in combination with 3-Amino-Phenyl-Borate (3-APB)
  • MBL: MIC determination of meropenem as a mono compound and in combination with the divalent cationic chelator EDTA
  • OXA-48-like type D-carbapenemases based on determination of high-level temocillin resistance
  • Low-level carbapenem resistance based on introduction of ertapenem / meropenem screening cut-off value according to EUCAST

MICRONAUT-S MDR MRGN-Screening

  • Resistance determination of all relevant gram-negative bacteria (Enterobacteria, Aeromonas, non-fermenters) against antibiotics and antibiotics combinations.
  • ceftazidime-avibactam and ceftolozane-tazobactam
  • MBL: MIC determination of meropenem as a mono substance and in combination with the divalent cationic chelator EDTA
  • KPC: MIC determination of meropenem as a mono substance and in combination with 3-Amino-Phenyl-Borate (3-APB)
  • Amp-C:  3 extended spectrum cephalosporins and their combination with (3-APB)
  • OXA-48-like type D carbapenemases based on the determination of temocillin high-level resistance
  • Low-level carbapenem resistance by introduction of meropenem screening concentrations